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Laboratory Description

Headquarters is in Sesto Fiorentino, within the framework of the "Florentine University Incubator".

It comprises approximately about 90 sq. m., including laboratory and office spaces.

The laboratory is equipped with sophisticated instrumentation, useful to perform pharmacodynamics, pharmacokinetics, biodistribution and toxicological preclinical in vitro analysis and to produce high-quality antibodies.

 Description
Model 
Provider 
AUTOMATED PATCH CLAMP SYSTEM 
IonFlux 16 
Molecular Devices 
SYSTEM FOR PURIFICATION OF PROTEINS
AKTA Pure 25L
GE Healthcare
FERMENTOR/BIOREACTOR FOR CELL CULTURE 
 BioFlo115 
 Eppendorf 
TABLETOP REFRIGERATED CENTRIFUGE
Allegra X-30R
Beckman Coulter
HIGH-PERFORMANCE CENTRIFUGE 
Avanti J-E 
Beckman Coulter 
HUMIDIFIED CO2  INCUBATOR 
 MCO-15AC 
 Sanyo 
BIOHAZARD CELL CULTURE CABINET 
 VBH36C2 
 Steril 
ELISA ABSORBANCE MICROPLATE READER LETTORE ELISA (controlled by Gen5™ software)
ELx800
Bio-Tek Instruments
SINGLE-CHANNEL MANUAL PIPETTE PIPET-LITE™ XLS SET
Rainin
OPTICAL MICROSCOPE
EVOS XL
AMG
LIQUID NITROGEN DEWAR
LS750B-R
Taylor-Wharton
ULTRA LOW TEMPERATURE FREEZER (-80°C)
K56PL
Beckman Coulter
MICRO-ARRAY SCANNER
 G2565BA
 Agilent Technologies 
LABORATORY REFRIGERATOR
 BLCRF290 
 Evermed
LABORATORY FREEZER
 BLCRF290
 Evermed 
BIOANALYZER 2100
G2939A
Agilent Technologies
NANOPHOTOMETER
NANODROP 2000
Thermo Scientific
RESEARCH PLUS PIPETTE SET
Eppendorf
MASTERCYCLER
Q-Cycler II Standard
Hain Lifescience
MASTERCYCLER
SI24
Hain Lifescience
HEATING MAGNETIC STIRRER
ARE
VELP Scientifica
INVERTED MICROSCOPE
PRIMO VERT
Zeiss
TRANSILLUMINATOR
TVL312R
Spectroline
ELECTROPHORESIS POWER SUPPLY + n. 2 ELECTROPHORETIC CELLS
EV245
Consort
N. 4 DATA LOGGERS
 
 
CHEMICAL HOOD
Green Airone 
Gelaire 
THERMOSTATI BATH
Certomat WR 
B.Braun Biotech 

Reference standards:

Description
Model
Provider
THERMOMETER
Bio-Temp 5182P100-qp
Alla France
TIMER
609-0190
Durac
ANALYTICAL BALANCE
SECURA120
Sartorius
PRECISION BALANCE
PRACTUM1100
Sartorius

 According to the agreements signed with the Experimental and Clinical Medicine Department and the Laboratory for the stabulation of animals of the University of Florence (Ce.S.A.L.), our researchers can perform in vivo experimental services, develop new preclinical models and produce transgenic mice.


The Ce.S.A.L. Animal facility comprises approximately 200 sq. m. with 5 rooms, and houses a mouse genetics lab, bioluminescence and fluorescence intra-vital imaging System (Photon Imager, BioSpace Lab) and state of the art equipment for measurements of haematological parameters.
In addition to the housing/experimentation area, this animal facility also contains one storage area dedicated to sterilisation.
Our staff also provides the basic animal breeding and maintenance services for genetically modified and wild type mouse strains.
 
Available equipment: Manual Patch Clamp System MULTICLAMP 700A (Axon Instruments); Optical Imager PHOTON IMAGER (BioSpace Lab.); Flow Cytometer FACSCantoI (Becton Dickinson), Microinjection System FEMTOJET (Eppendorf); Tabletop pH meter pH510 (Eutech Instruments); Tabletop Centrifuge (Eppendorf); Ultra low temperature freezer (Sanyo); Optical Microscope 100X (Leica); Biohazard cell cilture cabinets; Chemical hoods.